Calibration of a phase-shifting DIC microscope for quantitative phase imaging
Abstract
Phase-shifting differential interference contrast (DIC) provides images in which the intensity of DIC is transformed into values linearly proportional to differential phase delay. Linear regression analysis of the Fourier space, spiral phase, integration technique shows these values can be integrated and calibrated to provide accurate phase measurements of objects embedded in optically transparent media regardless of symmetry or absorption properties. This approach has the potential to overcome the limitations of profilometery, which cannot access embedded objects, and extend the capabilities of the traditional DIC microscope, which images embedded phase objects, but does not provide quantitative information.
Publication Title
Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Recommended Citation
King, S., Libertun, A., Preza, C., & Cogswell, C. (2007). Calibration of a phase-shifting DIC microscope for quantitative phase imaging. Progress in Biomedical Optics and Imaging - Proceedings of SPIE https://doi.org/10.1117/12.725061
