"Computational optical sectioning microscopy using an engineered PSF wi" by Shuai Yuan and Chrysanthe Preza
 

Computational optical sectioning microscopy using an engineered PSF with reduced depth variability Proof of concept

Abstract

In this simulation study, an engineered point-spread function (PSF) with reduced depth variability (due to depth-induced aberrations) was applied to three-dimensional computational optical-sectioning microscopy (COSM) imaging to investigate its impact on image restoration. Intermediate synthetic images from PSF-engineered COSM in the presence of aberrations were computed and then processed using a depth-variant expectation maximization algorithm. The restored images were compared to images simulated for traditional COSM. Results show that PSF-engineered COSM achieves better image restoration than conventional COSM and has the potential to improve imaging in the presence of depth-induced aberrations. © 2012 IEEE.

Publication Title

Proceedings - International Symposium on Biomedical Imaging

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