Computational optical sectioning microscopy using an engineered PSF with reduced depth variability Proof of concept
In this simulation study, an engineered point-spread function (PSF) with reduced depth variability (due to depth-induced aberrations) was applied to three-dimensional computational optical-sectioning microscopy (COSM) imaging to investigate its impact on image restoration. Intermediate synthetic images from PSF-engineered COSM in the presence of aberrations were computed and then processed using a depth-variant expectation maximization algorithm. The restored images were compared to images simulated for traditional COSM. Results show that PSF-engineered COSM achieves better image restoration than conventional COSM and has the potential to improve imaging in the presence of depth-induced aberrations. © 2012 IEEE.
Proceedings - International Symposium on Biomedical Imaging
Yuan, S., & Preza, C. (2012). Computational optical sectioning microscopy using an engineered PSF with reduced depth variability Proof of concept. Proceedings - International Symposium on Biomedical Imaging, 1739-1742. https://doi.org/10.1109/ISBI.2012.6235916