Densitometric HPTLC analysis of cordifolioside A in Tinospora cordifolia and commercial formulations
Abstract
A densitometric HPTLC method for analysis of cordifolioside A both in 60% methanolic extract of Tinospora cordifolia and in a commercial formulation has been established and validated. Cordifolioside A was separated on aluminum-backed silica gel 60 F254 plates with chloroform-methanol 85:15 (%, v/v) as mobile phase. A compact band was obtained for cordifolioside A at R F 0.52 ± 0.03. The limits of detection (LOD) and quantification (LOQ) were 20.12 and 60.36 ng per band, respectively. The highly precise and accurate method was used for analysis of cordifolioside A. © 2009 Akadémiai Kiadó, Budapest.
Publication Title
Acta Chromatographica
Recommended Citation
Alam, P., Ali, M., Singh, R., & Shakeel, F. (2009). Densitometric HPTLC analysis of cordifolioside A in Tinospora cordifolia and commercial formulations. Acta Chromatographica (4), 683-692. https://doi.org/10.1556/AChrom.21.2009.4.13
