Experimental implementation of wavefront encoding in 3D widefield fluorescence microscopy using a fabricated phase mask designed to reduce system depth variability

Abstract

Wavefront encoded (WFE) three dimensional fluorescent imaging is studied with a fabricated squared cubic (SQUBIC) phase mask. Experimental results demonstrate that, the WFE system can produce results predicted by simulations, and intermediate WFE images show 3% variability over a 60 μm imaging depth. © OSA 2016.

Publication Title

Optics InfoBase Conference Papers

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