Quantitative phase and amplitude imaging using Differential-Interference Contrast (DIC) microscopy
Abstract
We present an extension of the development of an alternating minimization (AM) method1 for the computation of a specimenüs complex transmittance function (magnitude and phase) from DIC images. The ability to extract both quantitative phase and amplitude information from two rotationally-diverse DIC images (i.e., acquired by rotating the sample) extends previous efforts in computational DIC microscopy that have focused on quantitative phase imaging only. Simulation results show that the inverse problem at hand is sensitive to noise as well as to the choice of the AM algorithm parameters. The AM framework allows constraints and penalties on the magnitude and phase estimates to be incorporated in a principled manner. Towards this end, Green and De Pierroüs "log-cosh" regularization penalty is applied to the magnitude of difierences of neighboring values of the complex-valued function of the specimen during the AM iterations. The penalty is shown to be convex in the complex space. A procedure to approximate the penalty within the iterations is presented. In addition, a methodology to pre-compute AM parameters that are optimal with respect to the convergence rate of the AM algorithm is also presented. Both extensions of the AM method are investigated with simulations. © 2009 SPIE.
Publication Title
Proceedings of SPIE - The International Society for Optical Engineering
Recommended Citation
Preza, C., & OüSullivan, J. (2009). Quantitative phase and amplitude imaging using Differential-Interference Contrast (DIC) microscopy. Proceedings of SPIE - The International Society for Optical Engineering https://doi.org/10.1117/12.819929
