Quantitative phase microscopy through differential interference imaging
Abstract
An extension of Nomarski differential interference contrast microscopy enables isotropic linear phase imaging through the combination of phase shifting, two directions of shear, and Fourier space integration using a modified spiral phase transform. We apply this method to simulated and experimentally acquired images of partially absorptive test objects. A direct comparison of the computationally determined phase to the true object phase demonstrates the capabilities of the method. Simulation results predict and confirm results obtained from experimentally acquired images. © 2008 Society of Photo-Optical Instrumentation Engineers.
Publication Title
Journal of Biomedical Optics
Recommended Citation
King, S., Libertun, A., Piestun, R., Cogswell, C., & Preza, C. (2008). Quantitative phase microscopy through differential interference imaging. Journal of Biomedical Optics (2) https://doi.org/10.1117/1.2907328
