Involvement of colchicine binding site of tubulin in the polymerisation process

Authors

D. Dasgupta, Bhabha Atomic Research Centre
R. Rajgopalan, Bhabha Atomic Research Centre
S. Gurnani, Bhabha Atomic Research Centre

Abstract

Role of lysine residues in the colchicine binding site and in the assembly-disassembly process was examined. It was observed that at 4°C (pH 7.5-8, 8±1) lysine residues and the N-terminal methionine residue of tubulin were all buried within the molecule. Evidence indicates that ε{lunate}-amino groups of lysine residues of tubulin are shared by both the colchicine binding site and the polymerisation process. © 1983.

Publication Title

FEBS Letters

Recommended Citation

Dasgupta, D., Rajgopalan, R., & Gurnani, S. (1983). Involvement of colchicine binding site of tubulin in the polymerisation process. FEBS Letters, 152 (1), 101-104. https://doi.org/10.1016/0014-5793(83)80491-8