Detection of immunoglobulin light-chain mRNA in lymphoid tissues using a practical in situ hybridization method
Abstract
The identification of immunoglobulin protein in routinely fixed and paraffin-embedded sections using antibodies combined with immunoperoxidase or similar techniques of detection is often problematic. We developed an in situ hybridization methodology for the identification of light-chain mRNA that is applicable to formalin-fixed, paraffin-embedded tissues, using neither radiolabelled or biotinylated oligonucleotide probes based on the kappa and lambda light-chain gene-constant regions. Reactive plasma cells can be consistently identified in reactive lymphoid tissues, and a monotypic pattern of light-chain mRNA restriction was seen in each of eight cases of multiple myeloma/plasmacytoma. Immunoblasts and germinal center cells also are labeled in reactive lymphoid tissues. Using 35S-labeled probes, 29 of 93 cases (30%) of non-Hodgkin's lymphomas had detectable light-chain mRNA, while 19% of non-Hodgkin's lymphomas were positive using biotinylated probes.
Publication Title
American Journal of Pathology
Recommended Citation
Weiss, L., Movamhed, L., Chen, Y., Shin, S., Stroup, R., Bui, N., Estess, P., & Bindl, J. (1990). Detection of immunoglobulin light-chain mRNA in lymphoid tissues using a practical in situ hybridization method. American Journal of Pathology, 137 (4), 979-988. Retrieved from https://digitalcommons.memphis.edu/facpubs/18510
