Cis-2-Decenoic Acid and Bupivacaine Delivered from Electrospun Chitosan Membranes Increase Cytokine Production in Dermal and Inflammatory Cell Lines

Authors

Zoe Harrison, Department of Biomedical Engineering, University of Memphis, Memphis, TN 38152, USA.
Emily C. Montgomery, Department of Biomedical Engineering, University of Memphis, Memphis, TN 38152, USA.
Joshua R. Bush, Department of Biomedical Engineering, University of Memphis, Memphis, TN 38152, USA.
Nidhi Gupta, Department of Biomedical Engineering, University of Memphis, Memphis, TN 38152, USA.
Joel D. Bumgardner, Department of Biomedical Engineering, University of Memphis, Memphis, TN 38152, USA.
Tomoko Fujiwara, Department of Chemistry, University of Memphis, Memphis, TN 38152, USA.
Daniel L. Baker, Department of Chemistry, University of Memphis, Memphis, TN 38152, USA.
Jessica Amber Jennings, Department of Biomedical Engineering, University of Memphis, Memphis, TN 38152, USA.Follow

Abstract

Wound dressings serve to protect tissue from contamination, alleviate pain, and facilitate wound healing. The biopolymer chitosan is an exemplary choice in wound dressing material as it is biocompatible and has intrinsic antibacterial properties. Infection can be further prevented by loading dressings with cis-2-decenoic acid (C2DA), a non-antibiotic antimicrobial agent, as well as bupivacaine (BUP), a local anesthetic that also has antibacterial capabilities. This study utilized a series of assays to elucidate the responses of dermal cells to decanoic anhydride-modified electrospun chitosan membranes (DA-ESCMs) loaded with C2DA and/or BUP. Cytocompatibility studies determined the toxic loading ranges for C2DA, BUP, and combinations, revealing that higher concentrations (0.3 mg of C2DA and 1.0 mg of BUP) significantly decreased the viability of fibroblasts and keratinocytes. These high concentrations also inhibited collagen production by fibroblasts, with lower loading concentrations promoting collagen deposition. These findings provide insight into preliminary cellular responses to DA-ESCMs and can guide future research on their clinical application as wound dressings.

Publication Title

Pharmaceutics

Recommended Citation

Harrison, Z., Montgomery, E. C., Bush, J. R., Gupta, N., Bumgardner, J. D., Fujiwara, T., Baker, D. L., & Jennings, J. A. (2023). Cis-2-Decenoic Acid and Bupivacaine Delivered from Electrospun Chitosan Membranes Increase Cytokine Production in Dermal and Inflammatory Cell Lines. Pharmaceutics, 15 (10) https://doi.org/10.3390/pharmaceutics15102476