Cloned ribosomal RNA genes from chloroplasts of Euglena gracilis

Authors

Margaret I. Lomax, University of Michigan, Ann Arbor
Robert B. Helling, University of Michigan, Ann Arbor
Lanny I. Hecker, Oak Ridge National Laboratory
Steven D. Schwartzbach, Oak Ridge National Laboratory

Abstract

Fragments of Euglena chloroplast DNA generated by endonuclease R·Eco RI were separated by agarose-gel electrophoresis into 24 distinct bands. At least five fragments contain sequences complementary to chloroplast ribosomal RNA. Most of the Eco RI fragments have been cloned in a plasmid of Escherichia coli. Three of the cloned fragments were shown to contain chloroplast ribosomal RNA sequences by DNA-RNA hybridization.

Publication Title

Science

Recommended Citation

Lomax, M., Helling, R., Hecker, L., & Schwartzbach, S. (1977). Cloned ribosomal RNA genes from chloroplasts of Euglena gracilis. Science, 196 (4286), 202-205. https://doi.org/10.1126/science.403604