Cloned ribosomal RNA genes from chloroplasts of Euglena gracilis

Abstract

Fragments of Euglena chloroplast DNA generated by endonuclease R·Eco RI were separated by agarose-gel electrophoresis into 24 distinct bands. At least five fragments contain sequences complementary to chloroplast ribosomal RNA. Most of the Eco RI fragments have been cloned in a plasmid of Escherichia coli. Three of the cloned fragments were shown to contain chloroplast ribosomal RNA sequences by DNA-RNA hybridization.

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