Cloned ribosomal RNA genes from chloroplasts of Euglena gracilis
Fragments of Euglena chloroplast DNA generated by endonuclease R·Eco RI were separated by agarose-gel electrophoresis into 24 distinct bands. At least five fragments contain sequences complementary to chloroplast ribosomal RNA. Most of the Eco RI fragments have been cloned in a plasmid of Escherichia coli. Three of the cloned fragments were shown to contain chloroplast ribosomal RNA sequences by DNA-RNA hybridization.
Lomax, M., Helling, R., Hecker, L., & Schwartzbach, S. (1977). Cloned ribosomal RNA genes from chloroplasts of Euglena gracilis. Science, 196 (4286), 202-205. https://doi.org/10.1126/science.403604