Disruption of the Saccharomyces Cerevlsiae gene for NADPH-cytochrome P450 reductase causes increased sensitivity to ketoconazole

Authors

Thomas R. Sutter, University of Cincinnati College of Medicine
John C. Loper, University of Cincinnati College of Medicine

Abstract

Strains of Saccharomyces cerevisiae deleted in the NADPH-cytochrome P450 reductase gene by transplacement are 200-fold more sensitive to ketoconazole, an inhibitor of the cytochrome P450 lanosterol 14α-demethylase. Resistance is restored through complementation by the plasmid-borne wild type gene from either S. cerevisiae or Candida tropicalis. Neither Southern hybridization nor Western immunoblot techniques provided evidence for a second NADPH-cytochrome P450 reductase gene, suggesting that an alternate pathway may provide for the functions of this reductase in S. cerevisiae. © 1989 Academic Press, Inc.

Publication Title

Biochemical and Biophysical Research Communications

Recommended Citation

Sutter, T., & Loper, J. (1989). Disruption of the Saccharomyces Cerevlsiae gene for NADPH-cytochrome P450 reductase causes increased sensitivity to ketoconazole. Biochemical and Biophysical Research Communications, 160 (3), 1257-1266. https://doi.org/10.1016/S0006-291X(89)80139-1