Consequences of Cre-mediated deletion of Ciz1 exon 5 in mice

Authors

Jianfeng Xiao, University of Tennessee Health Science CenterFollow
Mohammad Moshahid Khan, University of Tennessee Health Science CenterFollow
Satya Vemula, University of Tennessee Health Science Center
Jun Tian, University of Tennessee Health Science Center
Mark S. LeDoux, University of Tennessee Health Science CenterFollow

Abstract

CIZ1 plays a role in DNA synthesis at the G1/S checkpoint. Ciz1 gene-trap null mice manifest motor dysfunction, cell-cycle abnormalities, and DNA damage. In contrast, it has previously been reported that mouse embryonic fibroblasts derived from presumed Ciz1 knock-out mice (Ciz1tm1.1Homy/tm1.1Homy) generated by crossing Cre-expressing mice with exon 5-floxed mice (Ciz1tm1Homy/tm1Homy) do not exhibit evidence of enhanced DNA damage following γ-irradiation or cell-cycle defects. Here, we report that Ciz1tm1.1Homy/tm1.1Homy mice show loss of Ciz1 exon 5 but are neurologically normal and express abnormal transcripts (Ciz1ΔE5/ΔE5 mice) that are translated into one or more proteins of approximate wild-type size. Therefore, Ciz1tm1.1Homy/tm1.1Homy mice (Ciz1ΔE5/ΔE5) lose residues encoded by exon 5 but may gain function from novel amino acid sequences.

Publication Title

FEBS Letters

Recommended Citation

Xiao, J., Khan, M., Vemula, S., Tian, J., & LeDoux, M. (2018). Consequences of Cre-mediated deletion of Ciz1 exon 5 in mice. FEBS Letters, 592 (18), 3101-3110. https://doi.org/10.1002/1873-3468.13221