Molecular cloning and expression of rat torsinA in the normal and genetically dystonic (dt) rat

Authors

Philipp Ziefer, Massachusetts General Hospital
Joanne Leung, Massachusetts General Hospital
Tara Razzano, Massachusetts General Hospital
Christo Shalish, Massachusetts General Hospital
Mark S. LeDoux, Massachusetts General Hospital
Joan F. Lorden, Massachusetts General Hospital
Laurie Ozelius, Massachusetts General Hospital
Xandra O. Breakefield, Massachusetts General Hospital

Abstract

Deletions within the TOR1A gene cause early-onset (DYT1) torsion dystonia. We have cloned and sequenced the rat cDNA homologue of TOR1A and found a 91% identity with the human sequence. Northern blot analysis detects a single transcript of approximately 1.5 kb. In situ hybridization reveals a widespread distribution of torsinA mRNA within brain. No mutations were identified in the coding region of the gene in the genetically dystonic (dt) rat. © 2002 Elsevier Science B.V. All rights reserved.

Publication Title

Molecular Brain Research

Recommended Citation

Ziefer, P., Leung, J., Razzano, T., Shalish, C., LeDoux, M., Lorden, J., Ozelius, L., & Breakefield, X. (2002). Molecular cloning and expression of rat torsinA in the normal and genetically dystonic (dt) rat. Molecular Brain Research, 101 (1-2), 132-135. https://doi.org/10.1016/S0169-328X(02)00176-6