Anti-inflammatory effects of polyunsaturated fatty acids in THP-1 cells
Abstract
The effects of linoleic acid (LA), α-linolenic acid (ALA), and docosahexaenoic acid (DHA) were compared to that of palmitic acid (PA), on inflammatory responses in human monocytic THP-1 cells. When cells were pre-incubated with fatty acids for 2-h and then stimulated with lipopolysaccharide for 24-h in the presence of fatty acids, secretion of interleukin (IL)-6, IL-1β, and tumor necrosis factor-α (TNFα) was significantly decreased after treatment with LA, ALA, and DHA versus PA (P < 0.01 for all); ALA and DHA elicited more favorable effects. These effects were comparable to those for 15-deoxy-Δ12,14-prostaglandin J2 (15d-PGJ2) and were dose-dependent. In addition, LA, ALA, and DHA decreased IL-6, IL-1β, and TNFα gene expression (P < 0.05 for all) and nuclear factor (NF)-κB DNA-binding activity, whereas peroxisome proliferator-activated receptor-γ (PPARγ) DNA-binding activity was increased. The results indicate that the anti-inflammatory effects of polyunsaturated fatty acids may be, in part, due to the inhibition of NF-κB activation via activation of PPARγ. © 2005 Elsevier Inc. All rights reserved.
Publication Title
Biochemical and Biophysical Research Communications
Recommended Citation
Zhao, G., Etherton, T., Martin, K., Vanden Heuvel, J., Gillies, P., West, S., & Kris-Etherton, P. (2005). Anti-inflammatory effects of polyunsaturated fatty acids in THP-1 cells. Biochemical and Biophysical Research Communications, 336 (3), 909-917. https://doi.org/10.1016/j.bbrc.2005.08.204
