Effect of vitamin E (E) on expression of cell surface adhesion molecules and PGI2 production by human aortic endothelial cell (HAEC)

Abstract

Epidemiological and clinical studies indicate that E reduces the risk of cardiovascular disease (CVD). Modulation of adhesion molecules and prostanoids production by E may contribute to its beneficial effect. Previously we reported that E (α-tocopherol, α-T) reduced production of soluble ICAM-1 by HAEC dose dependency. Here, we report the effect of α-T on HAEC expression of cell surface ICAM-1, VCAM-1 and E selectin, and PGI2 release. The rank order expression of constitutive adhesion molecules by HAEC was ICAM-1 > VCAM-1 > F. selection. Incubation with IL-1β (20 ng/mL, 6 h) increased expression of ICAM-1 by 2x, VCAM-1 by 41x and E-selectin by 99x. Pre incubation of confluent HAEC with α-T at 20, 40 and 60 uM for 24 h decreased the consitutive expression of YCAM-1 but not the others. Enrichment of HAEC with 20, 40 and 60 uM α-T respectively suppressed IL-1β stimulated exppression of ICAM-1 by 33, 45 and 42%; VCAM-1 by 47, 75 and 80%, and E-selection by 25, 49 and 47%. Basal and IL-1β-stimulated PGI2 production was increased by increasing concentraation of α-T. Despite the increase in PGI2 prociaction, α-T dose-dependently decreased cyclooxygenase activity in IL-1β stimtated cells. It is concluded that α-T has differential effects on constitutive and stimulated expression of various adhesion molecules by HAEC. The α-T induced suppression in expression of adhesion molecules and increase in PCI2 production by HEAC may contribute to its reported reduction on risk of CVD.

Publication Title

FASEB Journal

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