Electronic Theses and Dissertations

Identifier

4896

Date

2017

Document Type

Thesis

Degree Name

Master of Science

Major

Biomedical Engineering

Committee Chair

Warren O Haggard

Committee Member

Jessica Amber Jennings

Committee Member

Harry S Courtney

Committee Member

Joel D Bumgardner

Abstract

Increases in patients' costs, multiple surgeries, and difficult treatment solutions are resultants of musculoskeletal infections. The presence of biofilm-forming or antibiotic resistant bacteria exponentially increase the complexity and complications in treatment of those musculoskeletal infections. During this study, the combination of ciprofloxacin and rifampin loaded in and released from chitosan-based local delivery systems was evaluated as an adjuvant therapy for prompt reduction of biofilm-forming bacteria in the wound when locally delivered. Primary assessments included antibiotic release, sponge eluate in vitro activity, in vitro synergy assays, effect on chitosan sponge pore structure, and an in vivo implant associated biofilm functional model. Antibiotic activity was present through seven days against S. aureus and P. aeruginosa. Ciprofloxacin had a therapeutic elution profile that lasted, at least, seven days while rifampin's lasted three days. Additive effects were present against P. aeruginosa during the in vitro synergy assay with inconclusive results against S. aureus. No unexpected or adverse effects on chitosan sponge pore structure were seen after sponges were loaded with the antibiotic cocktail. Complete clearance of biofilm-forming S. aureus and E. coli with no noticeable adverse effects were achieved in the functional infected pin murine model. The results of this study support the potential use of ciprofloxacin and rifampin in chitosan-based local deiivery devices, as local adjunctive therapy, for prevention of musculoskeletal and surgical site infections.

Comments

Data is provided by the student.

Library Comment

Dissertation or thesis originally submitted to the local University of Memphis Electronic Theses & dissertation (ETD) Repository.

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