Characterization of non-crosshybridizing DNA oligonucleotides manufactured in Vitro

Authors

Junghuei Chen, University of Delaware
Russell Deaton, University of ArkansasFollow
Max H. Garzon, University of Memphis
Jin Woo Kim, University of ArkansasFollow
David Wood, University of Delaware
Hong Bi, University of DelawareFollow
Dylan Carpenter, University of ArkansasFollow
Yu Zhen Wang, University of Delaware

Abstract

Libraries of DNA oligonucleotides manufactured by an In Vitro selection protocol were characterized for their non-crosshybridizing properties. Cloning and sequencing after several iterations of the protocol showed that the sequences, in general, became more non-crosshybridizing. Gel electrophoresis of protocol product, also, indicated non-crosshybridization, and showed evolution in the population of molecules under the non-crosshybridization selection pressure. Melting curves of protocol product also indicated non-crosshybridization when compared to control samples. Thus, it appears that the protocol does select populations of non-crosshybridizing sequences. © Springer-Verlag Berlin Heidelberg 2005.

Publication Title

Lecture Notes in Computer Science

Recommended Citation

Chen, J., Deaton, R., Garzon, M., Kim, J., Wood, D., Bi, H., Carpenter, D., & Wang, Y. (2005). Characterization of non-crosshybridizing DNA oligonucleotides manufactured in Vitro. Lecture Notes in Computer Science, 50-61. https://doi.org/10.1007/11493785_5