Characterization of non-crosshybridizing DNA oligonucleotides manufactured in Vitro
Abstract
Libraries of DNA oligonucleotides manufactured by an In Vitro selection protocol were characterized for their non-crosshybridizing properties. Cloning and sequencing after several iterations of the protocol showed that the sequences, in general, became more non-crosshybridizing. Gel electrophoresis of protocol product, also, indicated non-crosshybridization, and showed evolution in the population of molecules under the non-crosshybridization selection pressure. Melting curves of protocol product also indicated non-crosshybridization when compared to control samples. Thus, it appears that the protocol does select populations of non-crosshybridizing sequences. © Springer-Verlag Berlin Heidelberg 2005.
Publication Title
Lecture Notes in Computer Science
Recommended Citation
Chen, J., Deaton, R., Garzon, M., Kim, J., Wood, D., & Bi, H. (2005). Characterization of non-crosshybridizing DNA oligonucleotides manufactured in Vitro. Lecture Notes in Computer Science, 3384, 50-61. https://doi.org/10.1007/11493785_5
