Design and test of noncrosshybridizing oligonucleotide building blocks for DNA computers and nanostructures
Abstract
Design and test of noncrosshybridizing oligonucleotide building blocks for DNA computers and nanostructures were presented. A software design tool which used thermodynamic models of DNA duplex thermal stability and algorithms from graph theory, was developed. Noncrosshybridization and secondary structure were measured by running the annealing products on TAE polyacrylamide gels containing 10-mM Mg2+ at 4°C. An example set was tested and it was found that the designed sequences formed no unplanned duplexes and had no detectable secondary structure.
Publication Title
Applied Physics Letters
Recommended Citation
Deaton, R., Kim, J., & Chen, J. (2003). Design and test of noncrosshybridizing oligonucleotide building blocks for DNA computers and nanostructures. Applied Physics Letters (8), 1305-1307. https://doi.org/10.1063/1.1556557
