In situ fluorescence microscopy visualization and characterization of nanometer-scale carbon nanotubes labeled with 1-pyrenebutanoic acid, succinimidyl ester
Abstract
In order to characterize hybrid bio/abio technology utilizing carbon nanotubes (CNTs), in situ, real-time, yet noninvasive methods of accurate and reliable imaging are needed for observing CNTs' interactions with biological materials, i.e., DNA, in biologically relevant aqueous environments. Optical visualization and characterization of individual CNTs in aqueous solutions were explored in this study using 1-pyrenebutanoic acid, succinimidyl ester (PSE) and a conventional fluorescence microscope. The results demonstrate the potential of fluorescence microscopy based on PSE-based staining methodology monitoring with nanometer resolution of individual CNTs and their manipulation with biological materials in bio/abio hybrid systems. © 2006 American Institute of Physics.
Publication Title
Applied Physics Letters
Recommended Citation
Kim, J., Kotagiri, N., Kim, J., & Deaton, R. (2006). In situ fluorescence microscopy visualization and characterization of nanometer-scale carbon nanotubes labeled with 1-pyrenebutanoic acid, succinimidyl ester. Applied Physics Letters (21) https://doi.org/10.1063/1.2206875