Spatial light modulator phase mask implementation of wavefront encoded 3D computational-optical microscopy
Abstract
Spatial light modulator (SLM) implementation of wavefront encoding enables various types of engineered pointspread functions (PSFs), including the generalized-cubic and squared-cubic phase mask wavefront encoded (WFE) PSFs, shown to reduce the impact of sample-induced spherical aberration in fluorescence microscopy. This investigation validates dynamic experimental parameter variation of these WFE-PSFs. We find that particular design parameter bounds exist, within which the divergence of computed and experimental WFE-PSFs is of the same order of magnitude as that of computed and experimental conventional PSFs, such that model-based approaches for solving the inverse imaging problem can be applied to a wide range of SLM-WFE systems. Interferometric measurements were obtained to evaluate the SLM implementation of the numeric mask. Agreement between experiment and theory in terms of a wrapped phase, 0-2π, validates the phase mask implementation and allows characterization of the SLM response. These measurements substantiate experimental practice of computational-optical microscope imaging with an SLM-engineered PSF.
Publication Title
Applied Optics
Recommended Citation
King, S., Doblas, A., Patwary, N., Saavedra, G., Martínez-Corral, M., & Preza, C. (2015). Spatial light modulator phase mask implementation of wavefront encoded 3D computational-optical microscopy. Applied Optics (29), 8587-8595. https://doi.org/10.1364/AO.54.008587
